Plasma Flashlight Torches Bacteria

Plasma Flashlight Torches Bacteria

Handheld plasma flashlight rids skin of bacteria instantly

A group of Chinese and Australian scientists, including CSIRO, have developed a handheld, battery-powered plasma-producing device that can rid skin of bacteria in an instant.

The handheld plasma flashlight could be used in ambulance emergency calls, natural disaster sites, military combat operations and many other instances where treatment is required in remote locations.

Details of the plasma flashlight have been released today (5 April 2012) in a study published by the Institute of Physics Publishing’s Journal of Physics D: Applied Physics.

The flashlight is driven by a 12 volt battery and doesn’t require any external generator or wall power. It also doesn’t require any external gas feed or handling system. The device itself is fitted with resistors to stop it heating up and making it safe to touch.

“For individual bacteria, the inactivation time could be just tens of seconds.”

Prof Kostya (Ken) Ostrikov

In the study, the plasma flashlight effectively inactivated a thick biofilm of one of the most antibiotic and heat-resistant bacteria, Enterococcus faecalis – a bacterium which often infects the root canals during dental treatments.

Results showed that the plasma not only inactivated the top layer of cells, but penetrated deep into the very bottom of the layers to kill the bacteria.

Co-author of the study CSRIO’s Professor Kostya (Ken) Ostrikov, said: “The bacteria form thick biofilms, which makes them enormously resistant against inactivation which is extremely difficult to implement. High temperatures are commonly used but they would obviously burn our skin.

“In this study we chose an extreme example to demonstrate that the plasma flashlight can be very effective even at room temperature. For individual bacteria, the inactivation time could be just tens of seconds.”

“The device can be easily made and costs less than 100 US dollars to produce. Of course, some miniaturisation and engineering design may be needed to make it more appealing and ready for commercialisation,” Ostrikov added.

The temperature of the plume of plasma in the experiments was between 20-23 degrees C, which is very close to room temperature and therefore prevents any damage to the skin.

The biofilms were created by incubating the bacteria for seven days. The biofilms were around 25 micrometres thick and consisted of 17 different layers of bacteria. Each one was treated for five minutes with the plasma flashlight and then analysed to see how much of the bacteria survived.

Plasma – the fourth state of matter in addition to solids, liquids and gases – has previously shown its worth in the medical industry by effectively killing bacteria and viruses on the surface of the skin and in water.

Although the exact mechanism behind the anti-bacterial effect of plasma is largely unknown, it is thought that reactions between the plasma and the air surrounding it create a cocktail of reactive species that are similar to the ones found in our own immune system.

The researchers ran an analysis to see what species were present in the plasma and found that highly-reactive nitrogen and oxygen-related species dominated the results. Ultraviolet radiation has also been theorised as a reason behind plasma’s success; however, this was shown to be low in the jet created by the plasma flashlight, adding to the safety aspect of the device.

The device was created by an international team of researchers from Huazhong University of Science and Technology, CSIRO, The University of Sydney and the City University of Hong Kong.

Effective biofilm inactivation using a handheld, mobile plasma jet powered by a 12 V dc battery and operated in open air without any external gas supply is reported. This cold, room-temperature plasma is produced in self-repetitive nanosecond discharges with current pulses of ~100 ns duration, current peak amplitude of ~6 mA and repetition rate of ~20 kHz. It is shown that the reactive plasma species penetrate to the bottom layer of a 25.5 µm-thick Enterococcus faecalis biofilm and produce a strong bactericidal effect. This is the thickest reported biofilm inactivated using room-temperature air plasmas.

A collaborative team of scientists from several research institutes in China and Australia has developed a hand held, battery-powered plasma flashlight for removing bacteria from skin and other surfaces. The device was designed for use by paramedics or the military when treatment or sterilization is required in remote locations.

The device is self-contained and operates on a 12V battery without the need for an external gas feed or power. It generates a jet of plasma between 20-23°C (68-73.4°F), so that it won’t harm the skin and contains some protection circuitry to prevent overheating.

The researchers demonstrated the effectiveness of their prototype device by treating thick bacterial biofilms for up to five minutes with the plasma flashlight and measuring the bactericidal effect. Their results are published in the latest edition of Journal of Physics D: Applied Physics.

The research team has suggested that the device may cost as little as $100 to manufacture and a significant amount of miniaturization would be possible to reduce the size of their bulky prototype.

Source : http://www.csiro.au/Portals/Media/Handheld-plasma-flashlight.aspx

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